Química Nova (Jan 2013)

Determinação de nimesulida por análise por injeção em fluxo com detecção amperométrica de múltiplos pulsos

  • Amanda Barbosa Lima,
  • Sandro Cruz Chaves,
  • Leonardo Morais da Silva,
  • Polyana Fernandes Pereira,
  • Eduardo Mathias Richter,
  • Wallans Torres Pio dos Santos

DOI
https://doi.org/10.1590/S0100-40422013000900004
Journal volume & issue
Vol. 36, no. 9
pp. 1296 – 1302

Abstract

Read online

A simple and fast method for the determination of nimesulide (NI) using flow injection analysis with multiple-pulse amperometric (FIA-MPA) detection at a boron-doped diamond (BDD) electrode was developed. The method was based mainly on the application of a four-potential waveform, E1(det) = -0.8 V / 30 ms, E2(det) = 0.6 V / 30 ms, E3(det) = -0.4 V / 30 ms and E4(cleaning) = -0.45 V / 300 ms versus Ag/AgCl (3.0 mol L-1 KCl). NI was detected at three different electrode potentials, at which the nitro group undergoes different redox reactions. The proposed method was selective and sensitive (detection limit of 81.0 nmol L-1), and successfully applied for the determination of NI in pharmaceutical formulations, yielding similar results to those obtained by the reference method.

Keywords