Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant

Yong Zi Tan; Sriram Aiyer; Mario Mietzsch; Joshua A. Hull; Robert McKenna; Joshua Grieger; R. Jude Samulski; Timothy S. Baker; Mavis Agbandje-McKenna; Dmitry Lyumkis

doi:10.1038/s41467-018-06076-6

Nature Communications (Sep 2018)

Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant

Yong Zi Tan,
Sriram Aiyer,
Mario Mietzsch,
Joshua A. Hull,
Robert McKenna,
Joshua Grieger,
R. Jude Samulski,
Timothy S. Baker,
Mavis Agbandje-McKenna,
Dmitry Lyumkis

Affiliations

Yong Zi Tan: The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Sriram Aiyer: Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies
Mario Mietzsch: Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida
Joshua A. Hull: Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida
Robert McKenna: Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida
Joshua Grieger: Gene Therapy Center, University of North Carolina at Chapel Hill
R. Jude Samulski: Gene Therapy Center, University of North Carolina at Chapel Hill
Timothy S. Baker: Department of Chemistry and Biochemistry and Division of Biological Sciences, University of California-San Diego
Mavis Agbandje-McKenna: Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida
Dmitry Lyumkis: Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies

DOI: https://doi.org/10.1038/s41467-018-06076-6
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 11

Abstract

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Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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