Callus formation of sugar cane cultivar ‘SP 70-1284’ in liquid culture media starting from field explants

Abstract

Explants from immature sections of young leaves in the stems of the variety of sugarcane ‘SP 70-1284’, were taken directly of field whit the objets of study the capacity for organogenic callus formation. The effect of the concentration of 2,4-D in the callus formation was evaluated using liquid medium and considering the section of the explante utilized. The best callus formation and quality of them was achieved in the three first sections nearly to the apical meristem whit the use of 3 mg.l-1 of 2, 4-D. With these explantes the lowes indices of contamination and fenolization were obtained. Key words: callus, contamination, explant, fenolization, Saccharum sp.