Purification and composition of anthocyanins from red chicory

Abstract

Given the complex composition of red chicory crude extract, the content of impurities, low utilization rate and other issues, the pigment of red chicory was purified by porous microsphere method. Based on the raw material of red chicory, the adsorption and desorption properties of 8 kinds of porous microspheres to the anthodcyamins were compared to determine the best process conditions for purifying the anthocyanins of red chicory and the composition of the anthocyanins was analyzed by HPLC. The results show that the AB-8 type is the best purified microsphere, reaching adsorption balance at 2.5 h. When the pH value of the sample liquid is 2.0, the mass concentration is 341.33 mg/L and the volume is 260 mL, which is the best purification process condition, the volume fraction of desorption ethanol is 60% and the desorption is 1.5 h, the color value of the purified anthocyanins of red chicory pigment increased from 5.3 to 49.1, which is 9.3 times higher than the unpurified. The composition analysis show that the red chicory contains six kinds of anthocyanins, of which the content of chrysanthemum-3-O-glucoside is the highest.

Keywords

• purification of pigment
• red chicory
• porous microsphere
• hplc