The Apple Lipoxygenase <i>MdLOX3</i> Regulates Salt Tolerance and ABA Sensitivity

Xixia Chen; Daru Wang; Chunling Zhang; Xun Wang; Kuo Yang; Yongxu Wang; Xiaofei Wang; Chunxiang You

doi:10.3390/horticulturae8070651

Horticulturae (Jul 2022)

The Apple Lipoxygenase <i>MdLOX3</i> Regulates Salt Tolerance and ABA Sensitivity

Xixia Chen,
Daru Wang,
Chunling Zhang,
Xun Wang,
Kuo Yang,
Yongxu Wang,
Xiaofei Wang,
Chunxiang You

Affiliations

Xixia Chen: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Daru Wang: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Chunling Zhang: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Xun Wang: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Kuo Yang: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Yongxu Wang: Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, China
Xiaofei Wang: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Chunxiang You: National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China

DOI: https://doi.org/10.3390/horticulturae8070651
Journal volume & issue: Vol. 8, no. 7
p. 651

Abstract

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Various abiotic stresses, particularly salinization, restrict plant growth and yield around the world. Lipoxygenases play essential functions in coping with various stresses. In the present study, we found an apple (Malus domestica) homolog of Arabidopsis lipoxygenase3, named MdLOX3. MdLOX3 has a typical conserved lipoxygenase domain. MdLOX3 was expressed in all tissues of apple and was highly expressed in the root and flesh tissues by a qRT-PCR analysis. In addition, the promoter of MdLOX3 consists of multiple response elements. Various abiotic stresses and ABA treatment can induce the expression of MdLOX3. The overexpression of MdLOX3 in apple calli enhanced the ability to tolerate salt stress, and the heterotopic expression of MdLOX3 in Arabidopsis elevated salt stress tolerance via enhancing the ability of scavenging ROS. Furthermore, the overexpression of MdLOX3 in transgenic plants significantly reduced the sensitivity to ABA. Through the above, this work demonstrated that MdLOX3 played an active position in salt resistance and decreased the sensitivity to ABA, providing a theoretical reference for studying the role of MdLOX3 in abiotic stresses in apple.

Published in Horticulturae

ISSN: 2311-7524 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: http://www.mdpi.com/journal/horticulturae

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