LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p

Abstract

Yanan Zhang, Fang Ruan Department of Obstetrics and Gynecology, Affiliated Hospital of Jining Medical College, Jining 272000, People’s Republic of ChinaCorrespondence: Fang RuanDepartment of Obstetrics and Gynecology, Affiliated Hospital of Jining Medical College, 89 Guhuai Road, Jining, Shandong 272000, People’s Republic of ChinaEmail [email protected]: Growing evidence indicates that long noncoding RNA (lncRNA) is a group of important regulator in cancer development. However, the correlation between lncRNA and ovarian cancer remains elusive. Here, we aimed to investigate the roles of LEF1-AS1 in ovarian cancer progression.Methods: LEF1-AS1 expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Survival rate was analyzed by Kaplan-Meier method. Cell Counting Kit-8 (CCK8) and colony formation assays were used for proliferation analysis. Transwell assay was utilized for analyses of migration and invasion. Luciferase reporter assay was performed to test the interaction between LEF1-AS1 and miR-1285-3p.Results: We showed that LEF1-AS1 expression was upregulated in ovarian cancer tissues compared with normal tissues. Besides, LEF1-AS1 level was positively correlated with lymph node metastasis and advanced stage. Enhanced expression of LEF1-AS1 may predict a poor prognosis. Moreover, LEF1-AS1 knockdown suppressed ovarian cancer cell proliferation, migration and invasion. Mechanistically, LEF1-AS1 exerted its oncogenic functions through interacting with miR-1285-3p to inhibit miRNA activity. Rescue assay validated that miR-1285-3p inhibitors abrogated LEF1-AS1-silencer-caused suppression of ovarian cancer progression.Conclusion: Our study revealed that LEF1-AS1 acts as a vital regulation in ovarian cancer progression.Keywords: ovarian cancer, LEF1-AS1, miR-1285-3p, progression

Keywords

• ovarian cancer
• lef1-as1
• mir-1285-3p
• progression.