Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome

Valdir C. Barth; Ju-Mei Zeng; Irina O. Vvedenskaya; Ming Ouyang; Robert N. Husson; Nancy A. Woychik

doi:10.1038/s41467-019-10869-8

Nature Communications (Jul 2019)

Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome

Valdir C. Barth,
Ju-Mei Zeng,
Irina O. Vvedenskaya,
Ming Ouyang,
Robert N. Husson,
Nancy A. Woychik

Affiliations

Valdir C. Barth: Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School
Ju-Mei Zeng: Division of Infectious Diseases, Boston Children’s Hospital and Harvard Medical School
Irina O. Vvedenskaya: Waksman Institute, Rutgers University
Ming Ouyang: University of Massachusetts-Boston
Robert N. Husson: Division of Infectious Diseases, Boston Children’s Hospital and Harvard Medical School
Nancy A. Woychik: Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School

DOI: https://doi.org/10.1038/s41467-019-10869-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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MazF endoribonucleases are thought to arrest growth of Mycobacterium tuberculosis by global translation inhibition. Here, Barth et al. show that MazF-mt9 cleaves a specific tRNA, leading to ribosome stalling at AAA codons and thus selective mRNA degradation and changes in transcriptome and proteome.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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