Journal of Clinical and Diagnostic Research (Mar 2015)

Transit Fixatives: An Innovative Study

  • Rajanikanth M,
  • Ravi Prakash A,
  • Sreenath G,
  • Sonia Bai JK,
  • Shyam NDVN

DOI
https://doi.org/10.7860/JCDR/2015/11083.5615
Journal volume & issue
Vol. 9, no. 3
pp. ZM01 – ZM03

Abstract

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Background: Universally accepted fixative is 10% formalin which has been used for preserving the tissues and their architecture. In certain conditions, formalin might not be readily available for immediate fixation. We here by explore more economical, ecofriendly and easily available solutions that can be used as transit media/ transporting media for tissue specimens. Materials and Methods: The study included commonly available solutions like Spirit, Saline, Betadine solution, Hydrogen peroxide (H2 O2 ), Local anesthesia (L.A), Rose water, Coconut oil, Coconut water, Ice cold water, Honey and Milk while keeping formalin as control. The fresh tissue sample was cut into multiple bits and placed in different containers for a period of 8 hours before transferring to formalin solution. Conclusion: Transit fixatives are very important in certain situations where formalin is not readily available. These fixatives can be used to fix the tissues for a period of at least 8 hours without causing any damage or distortion before they are fixed in formalin solution.

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