Comparative sero evaluation of live and killed Gumboro vaccine in broilers.

Abstract

Infectious Bursal Disease (IBD) is a fatal disease, which is caused by a Birna virus. Vaccination is one of the most effectiveways to prevent virus. Two types of vaccines (live and killed) are normally used to induce immunity. In the presentexperiment, comparative sero evaluation of live and killed Gumboro vaccine in broilers was carried out. For comparingthe titers obtained with the two vaccines, one broiler group was vaccinated with live and the other with killed vaccine. Atfour points in time serum was collected from these broilers. Serum samples were collected on day 0 and day 14 of age(i.e. before vaccination was administered at 14 days of age) and subsequently on day 28 and day 42 (i.e. 14 and 28 daysafter vaccination). Collected serum samples were examined to determine antibody titer using the indirect ELISA method.The average maternal antibody titers were 2888.80 and 224.80 on day 0 and day 14 of age, respectively. The averagecombined titer of killed vaccine was 3582.1 on day 28 and day 42 and the average combined titer of live vaccine was1513 on days 28 and 42. The efficacy of killed vaccine was higher than that of live vaccine. It was observed that live vaccineinduced the desired immunity for a limited period of time. Higher antibody titers were obtained from killed vaccineafter live vaccine administration. For these reasons, it is suggested to administer live vaccine first and subsequently thekilled vaccine in order to obtain an immediate immune response and subsequently to maintain high antibody titers for along period.

Keywords

• Antibody titer, Live and killed vaccine, Infectious Bursal Disease, ELISA