A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

Vera Alexeeva; Sunita L. D'Souza; Christoph Schaniel

doi:10.1016/j.scr.2016.05.010

Stem Cell Research (Jul 2016)

A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

Vera Alexeeva,
Sunita L. D'Souza,
Christoph Schaniel

Affiliations

Vera Alexeeva: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States
Sunita L. D'Souza: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States
Christoph Schaniel: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States

DOI: https://doi.org/10.1016/j.scr.2016.05.010
Journal volume & issue: Vol. 17, no. 1
pp. 93 – 96

Abstract

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We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1+ cell populations.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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