Viruses (Jun 2018)

Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers

  • Brigitte Roy,
  • Cécile Philippe,
  • Martin J. Loessner,
  • Jacques Goulet,
  • Sylvain Moineau

DOI
https://doi.org/10.3390/v10060324
Journal volume & issue
Vol. 10, no. 6
p. 324

Abstract

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Applications for bacteriophages as antimicrobial agents are increasing. The industrial use of these bacterial viruses requires the production of large amounts of suitable strictly lytic phages, particularly for food and agricultural applications. This work describes a new approach for phage production. Phages H387 (Siphoviridae) and A511 (Myoviridae) were propagated separately using Listeria ivanovii host cells immobilised in alginate beads. The same batch of alginate beads could be used for four successive and efficient phage productions. This technique enables the production of large volumes of high-titer phage lysates in continuous or semi-continuous (fed-batch) cultures.

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