PLoS ONE (Jan 2015)

The PGE2/IL-10 Axis Determines Susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major Infection.

  • Angélica F Arcanjo,
  • Isabel F LaRocque-de-Freitas,
  • Juliana Dutra B Rocha,
  • Daniel Zamith,
  • Ana Caroline Costa-da-Silva,
  • Marise Pinheiro Nunes,
  • Fabio P Mesquita-Santos,
  • Alexandre Morrot,
  • Alessandra A Filardy,
  • Mario Mariano,
  • Christianne Bandeira-Melo,
  • George A DosReis,
  • Debora Decote-Ricardo,
  • Célio Geraldo Freire-de-Lima

DOI
https://doi.org/10.1371/journal.pone.0124888
Journal volume & issue
Vol. 10, no. 5
p. e0124888

Abstract

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B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. A mononuclear phagocyte derived from B-1 cells (B-1CDP) has been described. As the B-1CDP cells migrate to inflammatory/infectious sites and exhibit phagocytic capacity, the microbicidal ability of these cells was investigated using the Leishmania major infection model in vitro. The data obtained in this study demonstrate that B-1CDP cells are more susceptible to infection than peritoneal macrophages, since B-1CDP cells have a higher number of intracellular amastigotes forms and consequently release a larger number of promastigotes. Exacerbated infection by L. major required lipid bodies/PGE2 and IL-10 by B-1CDP cells. Both infection and the production of IL-10 were decreased when PGE2 production was blocked by NSAIDs. The involvement of IL-10 in this mechanism was confirmed, since B-1CDP cells from IL-10 KO mice are more competent to control L. major infection than cells from wild type mice. These findings further characterize the B-1CDP cells as an important mononuclear phagocyte that plays a previously unrecognized role in host responses to L. major infection, most likely via PGE2-driven production of IL-10.