Comparative analysis of biochemical changes at the systemic level in acute ischemia-reperfusion with morphological disorders of the soft tissues of the hind limbs of rats using neural network clustering

Abstract

Summary. Ischemia-reperfusion syndrome causes biochemical disorders and changes in the pro- and antioxidant system, both at the local and systemic levels. It is important to optimize the prediction of morphological disorders in relation to biochemical changes. The aim of the study – to establish the relationship between the state of lipid peroxidation and antioxidant protection at the systemic level and the degree of morphological changes in the soft tissues of the hind limbs of ruts under the development of ischemic-perfusion syndrome using neural network clustering. Materials and Methods. A biochemical study of the serum of 30 white rats was performed. There were 6 intact animals in the control group. Ischemia was modeled by applying SWAT rubber tourniquets on the hind limbs of the animal, at the level of the inguinal folds, for 2 hours, and reperfusion - by removing the tourniquet and restoring blood circulation 2 hours after its application. Observations of animals were carried out by train for 14 days. Blood for the study was isolated from the apex of the heart of the experimental animals by cardiotomy. Results. It was found that in the simulation of ischemia and reperfusion the largest deviations of the studied parameters are observed 1 day after removal of the tourniquet, namely a statistically significant increase in creatinine by 28.16 % (p

Keywords

• biochemical changes
• morphological changes
• serum
• ischemia-reperfusion
• neural network clustering