Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation

Atsushi Yoshizawa; Hiroto Egawa; Kimiko Yurugi; Rie Hishida; Hiroaki Tsuji; Eiji Ashihara; Aya Miyagawa-Hayashino; Satoshi Teramukai; Taira Maekawa; Hironori Haga; Sinji Uemoto

doi:10.1155/2013/972705

Clinical and Developmental Immunology (Jan 2013)

Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation

Atsushi Yoshizawa,
Hiroto Egawa,
Kimiko Yurugi,
Rie Hishida,
Hiroaki Tsuji,
Eiji Ashihara,
Aya Miyagawa-Hayashino,
Satoshi Teramukai,
Taira Maekawa,
Hironori Haga,
Sinji Uemoto

Affiliations

Atsushi Yoshizawa: Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
Hiroto Egawa: Department of Surgery, Institute of Gastroenterology, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
Kimiko Yurugi: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, Japan
Rie Hishida: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, Japan
Hiroaki Tsuji: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, Japan
Eiji Ashihara: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, Japan
Aya Miyagawa-Hayashino: Department of Diagnostic Pathology, Kyoto University, Kyoto 606-8507, Japan
Satoshi Teramukai: Division of Clinical Trial Design and Management, Translational Research Center, Kyoto University Hospital, Kyoto 606-8507, Japan
Taira Maekawa: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, Japan
Hironori Haga: Department of Diagnostic Pathology, Kyoto University, Kyoto 606-8507, Japan
Sinji Uemoto: Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan

DOI: https://doi.org/10.1155/2013/972705
Journal volume & issue: Vol. 2013

Abstract

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Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT.

Published in Clinical and Developmental Immunology

ISSN: 1740-2522 (Print); 1740-2530 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.hindawi.com/journals/jir/

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