Iranian Journal of Basic Medical Sciences (Dec 2015)

Evaluation of the circulating levels of IL-12 and IL-33 in patients with breast cancer: influences of the tumor stages and cytokine gene polymorphisms

  • Abdollah Jafarzadeh,
  • Kayhan Minaee,
  • Ali-Reza Farsinejad,
  • Maryam Nemati,
  • Arezu Khosravimashizi,
  • Hamid Daneshvar,
  • Mohammad Mehdi Mohammadi,
  • Abdolkarim Sheikhi,
  • Abbas Ghaderi

Journal volume & issue
Vol. 18, no. 12
pp. 1189 – 1198

Abstract

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Objective(s):IL-12 as an anti-tumor cytokine and IL-33 a novel identified cytokine with both pro- or anti-tumor activities, play important roles in response against tumor cells. Our aim was to evaluate the IL-12 and IL-33 levels and single nucleotide polymorphisms (SNP) in their genes in patients with breast cancer. Materials and Methods:Blood samples were collected from 100 patients with breast cancer, and 100 healthy women were controls. The serum IL-12 and IL-33 levels were measured by ELISA. The SNP rs3212227 (in IL-12 gene) and rs1929992 (in IL-33 gene) were determined using PCR-RFLP. Results:The IL-12 levels similarly expressed in patients and controls. IL-12 levels in patients at stage I were significantly lower than in the healthy group (P

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