Protocol for bacterial typing using Fourier transform infrared spectroscopy

Huayan Yang; Haimei Shi; Bin Feng; Li Wang; Liangqiang Chen; Avelino Alvarez-Ordóñez; Li Zhang; Hao Shen; Jianhua Zhu; Shouning Yang; Chuanfan Ding; Miguel Prietod; Fan Yang; Shaoning Yu

STAR Protocols (Jun 2023)

Protocol for bacterial typing using Fourier transform infrared spectroscopy

Huayan Yang,
Haimei Shi,
Bin Feng,
Li Wang,
Liangqiang Chen,
Avelino Alvarez-Ordóñez,
Li Zhang,
Hao Shen,
Jianhua Zhu,
Shouning Yang,
Chuanfan Ding,
Miguel Prietod,
Fan Yang,
Shaoning Yu

Affiliations

Huayan Yang: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Haimei Shi: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Bin Feng: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Li Wang: Kweichow Moutai Group, Renhuai, Guizhou 564501, China
Liangqiang Chen: Kweichow Moutai Group, Renhuai, Guizhou 564501, China
Avelino Alvarez-Ordóñez: Institute of Food Science and Technology, University of León, 24071 León, Spain
Li Zhang: Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
Hao Shen: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Jianhua Zhu: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China
Shouning Yang: Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Chuanfan Ding: Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
Miguel Prietod: Institute of Food Science and Technology, University of León, 24071 León, Spain; Corresponding author
Fan Yang: Kweichow Moutai Group, Renhuai, Guizhou 564501, China; Corresponding author
Shaoning Yu: Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China; Corresponding author

Journal volume & issue: Vol. 4, no. 2
p. 102223

Abstract

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Summary: The Fourier transform infrared (FT-IR) signals obtained from bacterial samples are specific and reproducible, making FT-IR an efficient tool for bacterial typing at a subspecies level. However, the typing accuracy could be affected by many factors, including sample preparation and spectral acquisition. Here, we present a unified protocol for bacterial typing based on FT-IR spectroscopy. We describe sample preparation from bacterial culture and FT-IR spectrum collection. We then detail FT-IR spectrum preprocessing and multivariate analysis of spectral data for bacterial typing. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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