Use of STS Marker Linked to ym4 Gene for the Genotyping of Winter Barleys

Abstract

The codominant STS marker MWG838, linked to BaYMV/BaMMV resistance gene ym4, was introduced into a winter barley breeding program. Lines of F2 and F3 progenies were created from crosses of the gene ym4 donor genotype Romanze and five different gene acceptors. Homozygous recessive, homozygous dominant, and heterozygous individuals were clearly identified and selected within F2 individuals by the used DNA marker linked to ym4 gene. F3 lines created by self-pollination from selected ym4ym4 F2 genotypes were reverified and included into further testing and breeding. The ym4 gene-linked presence of DNA marker was confirmed in progenies from all parental combinations.

Keywords

• winter barley
• baymv
• bammv
• ym4 gene
• marker assisted selection
• sts marker