Nature Communications (Jan 2022)
A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo
- Leo Hanke,
- Hrishikesh Das,
- Daniel J. Sheward,
- Laura Perez Vidakovics,
- Egon Urgard,
- Ainhoa Moliner-Morro,
- Changil Kim,
- Vivien Karl,
- Alec Pankow,
- Natalie L. Smith,
- Bartlomiej Porebski,
- Oscar Fernandez-Capetillo,
- Erdinc Sezgin,
- Gabriel K. Pedersen,
- Jonathan M. Coquet,
- B. Martin Hällberg,
- Ben Murrell,
- Gerald M. McInerney
Affiliations
- Leo Hanke
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Hrishikesh Das
- Department of Cell and Molecular Biology, Karolinska Institutet
- Daniel J. Sheward
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Laura Perez Vidakovics
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Egon Urgard
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Ainhoa Moliner-Morro
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Changil Kim
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Vivien Karl
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Alec Pankow
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Natalie L. Smith
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Bartlomiej Porebski
- Science for Life Laboratory, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet
- Oscar Fernandez-Capetillo
- Science for Life Laboratory, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet
- Erdinc Sezgin
- Science for Life Laboratory, Department of Women’s and Children’s Health, Karolinska Institutet
- Gabriel K. Pedersen
- Center for Vaccine Research, Statens Serum Institut
- Jonathan M. Coquet
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- B. Martin Hällberg
- Department of Cell and Molecular Biology, Karolinska Institutet
- Ben Murrell
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Gerald M. McInerney
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- DOI
- https://doi.org/10.1038/s41467-021-27610-z
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 11
Abstract
Here, the authors isolate and characterize a bispecific monomeric nanobody that induces dimerization of SARS-CoV-2 spike trimers, neutralizes variants of concerns as well as SARS-CoV, and inhibits SARS-CoV-2 infection in mice.
