Establishment of a Vietnamese ethnicity induced pluripotent stem cell line (VRISGi001-A) from umbilical cord blood hematopoietic stem cells under a feeder-free system

Abstract

We have established the original footprint-free Vietnamese human induced pluripotent stem cell line, VRISGi001-A, from cord-blood derived CD34 + hematopoietic stem cells (HSCs) of a 35 year old healthy woman under cGMP-compliant process. For the hiPSC induction, three Sendai virus vectors carrying four reprogramming factors including c-MYC, SOX2, KLF4, and OCT3/4 were delivered into CD34+ HSCs. The VRISGi001-A cell line expresses the majority of the pluripotent markers and differentiate in vitro into derivatives of three germ layers. The availability of Vietnamese hiPSC line could contribute to the improvement of inadequate genetic diversity in the currently available hiPSC lines.

Keywords

• Vietnamese human induced pluripotent stem cell
• Yamanaka factor
• Sendai virus