Green Synthesis and Antibacterial Activity Studies of Silver Nanoparticles from the Aqueous Extracts of Euphorbia hirta

Bennet Rohan D.; Raji P.; Divya Kumar M.; Ratna Geethika Gandham; Kripu Sharma V; Keerthana D.; Karishma S.; Antony V. Samrot; Iyappan Petchi; Thirumurugan R.; Sajna Keeyari Purayil; Paulraj Ponnaiah; Jenifer Selvarani A.; Prakash P.

doi:10.22207/JPAM.14.1.31

Journal of Pure and Applied Microbiology (Mar 2020)

Green Synthesis and Antibacterial Activity Studies of Silver Nanoparticles from the Aqueous Extracts of Euphorbia hirta

Bennet Rohan D.,
Raji P.,
Divya Kumar M.,
Ratna Geethika Gandham,
Kripu Sharma V,
Keerthana D.,
Karishma S.,
Antony V. Samrot,
Iyappan Petchi,
Thirumurugan R.,
Sajna Keeyari Purayil,
Paulraj Ponnaiah,
Jenifer Selvarani A.,
Prakash P.

Affiliations

Bennet Rohan D.: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Raji P.: ORCiD; Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Divya Kumar M.: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Ratna Geethika Gandham: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Kripu Sharma V: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Keerthana D.: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Karishma S.: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Antony V. Samrot: ORCiD; Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
Iyappan Petchi: Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
Thirumurugan R.: Department of Transfusion Medicine, JIPMER, Puducherry - 605 006, India.
Sajna Keeyari Purayil: Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
Paulraj Ponnaiah: Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
Jenifer Selvarani A.: ORCiD; Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
Prakash P.: Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.

DOI: https://doi.org/10.22207/JPAM.14.1.31
Journal volume & issue: Vol. 14, no. 1
pp. 301 – 306

Abstract

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The aqueous extracts of Euphorbia hirta was used to synthesise silver nanoparticles using bioreduction method. The nanoparticles were characterised by UV Vis spectroscopic analysis, SEM, EDX, AFM, XRD analysis. The silver nanoparticles were also tested for antibacterial activity in Pseudomonas aeruginosa and Bacillus subtilis. The minimum inhibitory concentration for the synthesised nano particles were also tested against the two bacterial species for the least concentration of 0.5µg/mL. The swarming motility assay and protein leakage assay was also tested for the nanoparticle. The silver nanoparticles were found to be much effective.

Published in Journal of Pure and Applied Microbiology

ISSN: 0973-7510 (Print); 2581-690X (Online)
Publisher: Journal of Pure and Applied Microbiology
Country of publisher: India
LCC subjects: Science: Microbiology
Website: https://www.microbiologyjournal.org/

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