Panel Reactive Antibody Profiles of Patients on the Kidney Transplant Waiting List of Atatürk University

Abstract

INTRODUCTION: Human leukocyte antigen (HLA) sensitization can be caused by various events such as blood transfusions, pregnancy, or organ transplantations. The aim of this retrospective study was to screen panel reactive antibodies (PRA) in transplant candidates and determine the anti-HLA profiles of PRA-positive patients METHODS: : Serum samples from 40 patients who were diagnosed with chronic renal failure and had positive PRA screening test results were used to identify antibody types. Antibody profiles of 40 patients were evaluated using a microbead-based method (Luminex). Single antigen bead (SAB) assay was performed using the manufacturer-recommended procedure. Local ethics committee approval was obtained. RESULTS: The most frequently detected class I anti-HLA antibodies were B7 (8.7%), A2 (8%), and A24 (8%), and the most common class II anti-HLA antibodies were DR01 (11.67%), DR11 (11.67%), and DQ3 (16.70%). DISCUSSION AND CONCLUSION: Our results are similar to those of other studies on antigen frequencies in the population. However, we observed that certain antibodies (A23, A68, B15, B40, DR04, DR07, DR09, and DR16) were produced at high levels.

Keywords

• anti-hla
• class i
• class ii
• pra
• single antigen bead assay