Development of Quality Control Parameters for Standardization of Herb of the Cultivated Plant <i>Alchemilla mollis</i> (Buser) Rothm.

Abstract

Introduction. Alchemilla herb is proposed for introduction into pharmaceutical practice, for obtaining extracts with various pharmacological activity. To expand, resource base of Alchemilla it has been proposed to use a high biomass cultivated plant – Alchemilla mollis (Buuser) Rothm. In terms of qualitative and quantitative characteristics of the chemical composition Alchemilla mollis herb is comparable to the samples of raw materials of wild-growing Alchemilla. The presence of chemotaxonomic features necessitates the preparation of regulatory documentation for the introduction of this plant as a source of medicinal raw materials.Aim. Development of a methodology for assessing the main group of biologically active substances and the quantitative determination of flavonoids in Alchemilla mollis herb.Materials and methods. As objects of study, we used Alchemilla mollis herb harvested from plants cultivated in the Perm Krai. The chromatographic parameters of raw material authenticity were determined by thin layer chromatography (TLC). To develop the parameters for the quantitative determination of flavonoids in Alchemilla mollis herb, a modification of the method proposed for Alchemilla herb was carried out.Results and discussion. During chromatographic study of Alchemilla mollis herb were identified cinaroside, rutin, and quercetin. Cynaroside was referred to the marker substances. Ethyl acetate : acetic acid (85 : 15) was chosen as the optimal chromatographic system. A modification and validation of the method for the quantitative determination of flavonoids in Alchemilla herb was carried out. The change of the extractant, particle size, time and frequency of extraction for sample preparation and the optimal use of cynaroside as a standard substance are substantiated. The optimal conditions for the reaction of complex formation with aluminum chloride are established.Conclusion. To determine the authenticity of Alchemilla mollis herb, it was proposed to use the identification of cynaroside by TLC. The modified method for the quantitative determination of flavonoids in terms of cynaroside for the Alchemilla mollis herb, reproducible, correct and can be used for standardization. When testing the methodology on samples of raw materials harvested in the Perm region, a range of values for the content of flavonoids was 3.14–4.84 %, with an average level of variability.

Keywords

• <i>alchemilla mollis</i>
• thin layer chromatography (tlc)
• spectrophotometry
• flavonoids
• cynaroside