Frequency of SEN Virus in Cervicitis and Cervical Cancers in Isfahan

Abstract

Background: SEN virus is mainly transmitted via blood and its relationship with different diseases is under investigation. The prevalence of SEN-V in healthy individuals, including blood donors, clearly differs geographically. Female genital lesions range from mild infections without symptoms such as chronic cervicitis to various cancers. This study was done to investigate the possible correlation of SEN virus infection with cervicitis and cervical tumors. Methods: In this study, 117 samples of formalin fixed paraffin embedded biopsies of uterus and cervix were subjected to nested PCR to detect D and H strains of SEN-V. After extraction from gel, two PCR products were sequenced and compared with the sequences in Gene Bank. Fisher’s exact test was used for statistical analyses. Findings: High homology with D and H strains was observed. SEN-V was detected only in 4 cases of 45 cancer samples which one was positive for H, one for D and one for both strains. Out of 72 cervicitis cases 13 and 9 subjects were positive for D and H strains respectively. Compare to cervical cancers, the frequency of infection was higher in cervicitis cases (30%) (P > 0.05). Conclusion: May be there is a correlation between SEN-V infection with cervicitis, especially in chronic cases with unknown etiology which needs more studies to be proved.

Keywords

• SEN virus
• Cervicitis
• Uterus cancers
• Polymerase chain reaction (PCR)