Nature Communications (Jul 2020)
Strategies to enable large-scale proteomics for reproducible research
- Rebecca C. Poulos,
- Peter G. Hains,
- Rohan Shah,
- Natasha Lucas,
- Dylan Xavier,
- Srikanth S. Manda,
- Asim Anees,
- Jennifer M. S. Koh,
- Sadia Mahboob,
- Max Wittman,
- Steven G. Williams,
- Erin K. Sykes,
- Michael Hecker,
- Michael Dausmann,
- Merridee A. Wouters,
- Keith Ashman,
- Jean Yang,
- Peter J. Wild,
- Anna deFazio,
- Rosemary L. Balleine,
- Brett Tully,
- Ruedi Aebersold,
- Terence P. Speed,
- Yansheng Liu,
- Roger R. Reddel,
- Phillip J. Robinson,
- Qing Zhong
Affiliations
- Rebecca C. Poulos
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Peter G. Hains
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Rohan Shah
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Natasha Lucas
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Dylan Xavier
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Srikanth S. Manda
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Asim Anees
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Jennifer M. S. Koh
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Sadia Mahboob
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Max Wittman
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Steven G. Williams
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Erin K. Sykes
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Michael Hecker
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Michael Dausmann
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Merridee A. Wouters
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Keith Ashman
- Sciex, 2 Gilda Court
- Jean Yang
- School of Mathematics and Statistics, The University of Sydney
- Peter J. Wild
- Dr. Senckenberg Institute of Pathology, University Hospital Frankfurt
- Anna deFazio
- Centre for Cancer Research, Westmead Institute for Medical Research
- Rosemary L. Balleine
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Brett Tully
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Ruedi Aebersold
- Department of Biology, Institute of Molecular Systems Biology, ETH Zürich
- Terence P. Speed
- Bioinformatics Division, Walter and Eliza Hall Institute of Medical Research
- Yansheng Liu
- Department of Pharmacology, Yale University School of Medicine
- Roger R. Reddel
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Phillip J. Robinson
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- Qing Zhong
- ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney
- DOI
- https://doi.org/10.1038/s41467-020-17641-3
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Clinical proteomics critically depends on the ability to acquire highly reproducible data over an extended period of time. Here, the authors assess reproducibility over four months across different mass spectrometers and develop a computational approach to mitigate variation among instruments over time.
