Identification of Purity and Authenticity of Hybrid Seeds of 'Huifeng 2' Pepper Based on SSR Molecular Markers

Abstract

【Objective】'Huifeng 2' is an F1 hybrid variety which is highly favoured by farmers and consumers due to its characteristics of mid-early maturity, strong disease resistance, storage and transportation resistance and good quality. To ensure the quality of seeds, it intends to establish a set of quick and easy methods for seed purity and authenticity identification.【Method】Based on the publicly available SSR molecular markers of pepper genome, SSR markers with obvious polymorphisms, clear bands and stable amplification were screened by using the male and female parents of 'Huifeng 2' as materials. Then, the purity of the hybrid seeds of 'Huifeng 2' was identified, and the accuracy of the molecular marker was verified by using morphology identification in the field. In addition, the authenticity of four varieties, including 'Yuehong 1', 'Huifeng 1', 'Huifeng 2' and 'Huifeng 5', was identified with the screened markers.【Result】Two SSR markers, L0143 and L0622, were selected for their obvious polymorphism and clear bands with stable amplification. The PCR amplification bands of marker L0143 were 149 bp in the female parent W2280 and 141 bp in the male parent W2102, and the amplification bands of marker L0622 were 208 bp and 196 bp in the female parent and male parent, respectively. When used alone, the two markers could accurately identify the purity of 'Huifeng 2' seeds, and the simultaneous use of the two markers could distinguish the four varieties of 'Yuehong 1', 'Huifeng 1', 'Huifeng 2' and 'Huifeng 5', which could be used to identify their authenticity.【Conclusion】The SSR molecular markers L0143 and L0622 selected in this study can identify the purity and authenticity of 'Huifeng 2' hybrid seeds rapidly, accurately, and effectively with low-cost, which are simple to operate and can replace the traditional method of identifying the purity and authenticity of hybrid seeds. It has a high commercial application prospect.

Keywords

• pepper
• 'huifeng 2'
• seed purity
• ssr molecular marker
• authenticity identification