Mycorrhizal roles in plant growth, gas exchange, root morphology, and nutrient uptake of walnuts

Abstract

Walnut, an important oil fruit tree, is dependent on arbuscular mycorrhizas, while mycorrhizal roles and efficient mycorrhizal fungus in walnuts are unknown. This study was conducted to evaluate the effect of five arbuscular mycorrhizal fungi (AMF) species, including Acaulospora scrobiculata, Diversispora spurca, Glomus etunicatum, G. mosseae, and G. versiforme on plant growth, leaf gas exchange, root morphology, and root nutrient contents of walnut (Juglans regia L. Liaohe 1) seedlings. Three months of AMF inoculations later, root mycorrhizal colonisation achieved 47.0% to 76.4%. AMF treatments increased plant growth performance, dependent on AMF species. AMF-inoculated plants with D. spurca, G. etunicatum, and G. mosseae showed higher root length, projected area, surface area, and volume than non-AMF plants. Except for G. versiforme, the other four AMF treatments almost significantly increased leaf photosynthesis rate, transpiration rate, and stomatal conductivity, while reduced intercellular CO2 concentrations and leaf temperature. AMF affected root nutrient contents, dependent on AMF and mineral nutrient species. These results, thereby, concluded that AMF had a positive role in walnuts, dependent on AMF species, and D. spurca was the best mycorrhizal fungus for walnut. Such results provide the potential possibility of a developing consortium of AMF in walnut cultivation management.

Keywords

• endophytic fungi
• mineral nutrients
• mycorrhizal symbiosis
• rhizosphere
• root colonisation