Clinical, Cosmetic and Investigational Dermatology (Mar 2024)

Protective Effect of Panax notoginseng Extract Fermented by Four Different Saccharomyces cerevisiae Strains on H2O2 Induced Oxidative Stress in Skin Fibroblasts

  • Wang Z,
  • Fang J,
  • Zu S,
  • Sun Q,
  • Song Z,
  • Geng J,
  • Wang D,
  • Li M,
  • Wang C

Journal volume & issue
Vol. Volume 17
pp. 621 – 635

Abstract

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Ziwen Wang,1,2 Jiaxuan Fang,1,2 Shigao Zu,1,2 Qianru Sun,1,2 Zixin Song,1,2 Jiman Geng,1,2 Dongdong Wang,1,2 Meng Li,1,2 Changtao Wang1,2 1Beijing Key Laboratory of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, 100048, People’s Republic of China; 2School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, People’s Republic of ChinaCorrespondence: Dongdong Wang; Changtao Wang, Beijing Technology and Business University, Beijing, 100048, People’s Republic of China, Tel/Fax +86 158 1135 7756, Email [email protected]; [email protected]: To produce Panax notoginseng extract as a cosmetic ingredient through Saccharomyces cerevisiae fermentation.Methods: We first compared the total sugar content, polysaccharide content, reducing sugar content, total phenolic content, total saponin content, DPPH free radical, ABTS free radical, hydroxyl free radical scavenging ability and ferric reducing antioxidant power (FRAP) of Panax notoginseng fermented extract (pnFE) and unfermented extract (pnWE). Their potential correlations were analyzed by Pearson’s correlation analysis. Then, the oxidative stress model of H2O2-induced MSFs was used to evaluate the effects of different pnFE on MSF viability, reactive oxygen species (ROS), malondialdehyde (MDA), and the activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) to explore their protective effects on MSFs subjected to H2O2-induced cellular oxidative damage. Finally, their safety and stability were evaluated by using the red blood cell (RBC) test and hen’s egg test-chorioallantoic membrane (HET-CAM) assay, and changes in pH and content of soluble solids, respectively.Results: Compared with pnWE, pnFE has more active substances and stronger antioxidant capacity. In addition, pnFE has a protective effect on H2O2-induced oxidative stress in MSFs with appropriate safety and stability.Conclusion: PnFE has broad application prospects in the field of cosmetics.Keywords: Panax notoginseng, Saccharomyces cerevisiae, fermentation, oxidative stress, fibroblast cells, cosmetic ingredients

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