Pesticidi i Fitomedicina (Jan 2021)

Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper

  • Ristić Danijela,
  • Vučurović Ivan,
  • Aleksić Goran,
  • Nikolić Bogdan,
  • Đurović Sanja,
  • Starović Mira

DOI
https://doi.org/10.2298/PIF2102073R
Journal volume & issue
Vol. 36, no. 2
pp. 73 – 82

Abstract

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Application of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus), phototrophic bacteria (Rhodopseudomonas palustris) and yeast (Saccharomyces cerevisiae) with sugar cane molasses, marked as: EM1, EM5 and EM AGRO, against the phytopathogenic fungi of tomato and pepper: Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Colletotrichum sp., Verticilium dahliae and Pythium aphanidermatum was evaluated in vitro and in vivo. A combination of bacteria and yeast named EM5 showed the highest mycelium growth inhibition against B. cinerea (38.4%) in a double agar diffusion test. In a microdilution test, the combination EM1 showed the highest inhibitory effect on B. cinerea (MIC 1x10-3 ml/ml), while EM5 showed a similar inhibitory effect towards F. oxysporum, A. alternata and Colletotrichum sp. (MIC 10 ml/ml). The use of EM1 (in concentrations 10 and 100 µl/ml) and EM AGRO (10 µl/ml) is recommended for tomato seedling protection. EM1 (100 µl/ml), EM5 and EM AGRO (10 µl/ml) are recommended for pepper seedling protection.

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