Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul (Aug 2008)
COMPARISON OF SERUM LEVEL OF INTERLEUKIN (IL)-10 AND INTERLEUKIN (IL)-12 HUMAN BRUCELLOSIS IN CATTLE OWNERS AND HEALTH CONTROLS (BABOL)
Abstract
BACKGROUND AND OBJECTIVE: Human brucellosis is a worldwide zoonotic infectious disease that can be transmitted between animals and humans. Cattle owners who have occupational contact with animals are at the risk of this disease. Evaluation of production of cytokines especially IL-10 and IL-12 are important factor in investigation of immune response against a variety of intracellular pathogen, vaccination and other immune reactions. In the present study, the cytokines induced during this infection determined the level of IL-10 and IL-12 in serum of cattle owners with brucellosis and without brucellosis compared with health controls. METHODS: This cross-sectional and descriptive study was performed on 30 cattle owners with brucellosis and 30 cattle owners without brucellosis and 20 person of health control. Then 5cc blood sample have been taken from donors and cytokines IL-10 and IL-12 of ELISA were determined by Diaclone company. FINDINGS: The mean serum IL-10 level of cattle owners with positive brucellosis (133.6 ± 20.4) and negative brucellosis (55.5±29.2) compared with the health control (16.1±3.2). This difference was statistically significant (p<0.05). Considering the results of this investigation the mean IL-12 level in positive brucellosis cattle owners (16.6±14.7) and negative brucellosis (15.2±8.6) compared with the health control (3.7±0.8). However production of IL-12 response to brucella antigenic in positive brucellosis cattle owners and negative brucellosis was increased in comparison to the health control, differences between the values was not significant. CONCLUSION: Increase of IL-10 indicated relationship between brucellosis and Th2 cell response. Control of immune response can be effective in the treatment of disease.
