Selective and sensitive detection of hypochlorite in living cells using reduced iodine-doped carbon dots

Xinyue Zhou; Shiyi Zhang; Jian Lv; Xiaoyuan Wang; Lijuan Gong; Yating Gao; Shuai Chang; Bin Bin Chen; Dawei Li; Ruocan Qian

Next Materials (Jul 2024)

Selective and sensitive detection of hypochlorite in living cells using reduced iodine-doped carbon dots

Xinyue Zhou,
Shiyi Zhang,
Jian Lv,
Xiaoyuan Wang,
Lijuan Gong,
Yating Gao,
Shuai Chang,
Bin Bin Chen,
Dawei Li,
Ruocan Qian

Affiliations

Xinyue Zhou: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Shiyi Zhang: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Jian Lv: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Xiaoyuan Wang: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Lijuan Gong: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Yating Gao: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Shuai Chang: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Bin Bin Chen: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), 2001 Longxiang Boulevard, Longgang District, Shenzhen City, Guangdong 518172, China; Corresponding author at: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Dawei Li: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Ruocan Qian: Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; Corresponding author.

Journal volume & issue: Vol. 4
p. 100220

Abstract

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Hypochlorite (ClO−) is an important metabolite that participates a series of intracellular signal communications, which is closely related with the generation and development of various diseases. Herein, we design and prepare reduced iodine-doped carbon dots (I-CDs) that are highly sensitive to ClO−. The proposed I-CDs show bright green fluorescence (FL), which can be efficiently quenched upon treatment with ClO− because of the destroy of I-CDs structure caused by oxidation of iodine by ClO−. This sensing platform displays a good linear response range of 1 − 60 μM with a low detection limit of 95.1 nM. Importantly, monitoring ClO− level in living cells is further realized, demonstrating the capability of this strategy to determine the content of intracellular ClO−. This work provides a promising tool for visual sensing ClO−, which is of great significance for early diagnosis of ClO− related diseases.

Published in Next Materials

ISSN: 2949-8228 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology
Website: https://www.sciencedirect.com/journal/next-materials

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