Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line

Wei shan; Xiaomeng Cui; Dayan Wang; Baiqiang Wang; Xiangge Guo; Xumeng Wang; Jiaxuan Wang; Yanting Li; Guipeng An; Qian Ren

Stem Cell Research (Dec 2024)

Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line

Wei shan,
Xiaomeng Cui,
Dayan Wang,
Baiqiang Wang,
Xiangge Guo,
Xumeng Wang,
Jiaxuan Wang,
Yanting Li,
Guipeng An,
Qian Ren

Affiliations

Wei shan: Department of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; National Center for Clinical Medicine of Neurological Diseases, Beijing 10070, China
Xiaomeng Cui: Department of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Department of Neurology, Zibo City Hospital Combined of Traditional Chinese and Western Medicine, Zibo 255000, China
Dayan Wang: Department of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Department of Neurology, Avant-garde Hospital of Jilin Province, Changchun 130012, China
Baiqiang Wang: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 100034, China; Department of Cardiology, Qilu Hospital of Shandong University, Jinan 100034, China
Xiangge Guo: Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China
Xumeng Wang: Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China
Jiaxuan Wang: Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China
Yanting Li: Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China
Guipeng An: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 100034, China; Department of Cardiology, Qilu Hospital of Shandong University, Jinan 100034, China; Corresponding authors.
Qian Ren: Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, China; Corresponding authors.

Journal volume & issue: Vol. 81
p. 103596

Abstract

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The KCNA2 gene is the voltage-gated ion channel from both functional and structural perspectives. KCNA2 is involved in diverse functions including the regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. To investigate the relevant pathophysiological mechanisms, we generated heterozygous KCNA2 knockout human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 gene editing. The generated iPSCs had a normal karyotype, were free of genetically integrated epitaxial plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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