A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

Martin W. LaFleur; Thao H. Nguyen; Matthew A. Coxe; Kathleen B. Yates; Justin D. Trombley; Sarah A. Weiss; Flavian D. Brown; Jacob E. Gillis; Daniel J. Coxe; John G. Doench; W. Nicholas Haining; Arlene H. Sharpe

doi:10.1038/s41467-019-09656-2

Nature Communications (Apr 2019)

A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

Martin W. LaFleur,
Thao H. Nguyen,
Matthew A. Coxe,
Kathleen B. Yates,
Justin D. Trombley,
Sarah A. Weiss,
Flavian D. Brown,
Jacob E. Gillis,
Daniel J. Coxe,
John G. Doench,
W. Nicholas Haining,
Arlene H. Sharpe

Affiliations

Martin W. LaFleur: Department of Microbiology and Immunobiology, Harvard Medical School
Thao H. Nguyen: Department of Microbiology and Immunobiology, Harvard Medical School
Matthew A. Coxe: Department of Microbiology and Immunobiology, Harvard Medical School
Kathleen B. Yates: Department of Pediatric Oncology, Dana-Farber Cancer Institute
Justin D. Trombley: Department of Microbiology and Immunobiology, Harvard Medical School
Sarah A. Weiss: Department of Pediatric Oncology, Dana-Farber Cancer Institute
Flavian D. Brown: Department of Microbiology and Immunobiology, Harvard Medical School
Jacob E. Gillis: Department of Microbiology and Immunobiology, Harvard Medical School
Daniel J. Coxe: School of Energy, Matter, and Transport Engineering at Arizona State University
John G. Doench: Broad Institute of Harvard and Massachusetts Institute of Technology
W. Nicholas Haining: Department of Pediatric Oncology, Dana-Farber Cancer Institute
Arlene H. Sharpe: Department of Microbiology and Immunobiology, Harvard Medical School

DOI: https://doi.org/10.1038/s41467-019-09656-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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