Structural basis of ECF-σ-factor-dependent transcription initiation

Wei Lin; Sukhendu Mandal; David Degen; Min Sung Cho; Yu Feng; Kalyan Das; Richard H. Ebright

doi:10.1038/s41467-019-08443-3

Nature Communications (Feb 2019)

Structural basis of ECF-σ-factor-dependent transcription initiation

Wei Lin,
Sukhendu Mandal,
David Degen,
Min Sung Cho,
Yu Feng,
Kalyan Das,
Richard H. Ebright

Affiliations

Wei Lin: Waksman Institute and Department of Chemistry, Rutgers University
Sukhendu Mandal: Waksman Institute and Department of Chemistry, Rutgers University
David Degen: Waksman Institute and Department of Chemistry, Rutgers University
Min Sung Cho: Waksman Institute and Department of Chemistry, Rutgers University
Yu Feng: Waksman Institute and Department of Chemistry, Rutgers University
Kalyan Das: Rega Institute and Department of Microbiology and Immunology, KU Leuven
Richard H. Ebright: Waksman Institute and Department of Chemistry, Rutgers University

DOI: https://doi.org/10.1038/s41467-019-08443-3
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

Read online

No structural data have been available for RNA polymerase holoenzymes or transcription initiation complexes that contain extracytoplasmic σ factors. Here the authors report the crystal structures of transcription initiation complexes comprising Mycobacterium tuberculosis RNA polymerase, extracytoplasmic σ factor σL and promoter DNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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