An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissue

Abstract

Summary: Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample. Here, we present a ChIP protocol from murine inguinal white adipose tissue. We describe steps for tissue harvesting, crosslinking, chromatin extraction, shearing, immunoprecipitation, and purification. We then detail procedures for analysis including library preparation, sequencing, and qRT-PCR validation.For complete details on the use and execution of this protocol, please refer to Antonia Katsouda et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Sequence Analysis
• Genomics
• ChIP-seq
• Metabolism
• Molecular Biology