Frontiers in Immunology (Sep 2018)

CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes

  • Zachary L. Benet,
  • Matangi Marthi,
  • Fang Ke,
  • Rita Wu,
  • Jackson S. Turner,
  • Jahan B. Gabayre,
  • Michael I. Ivanitskiy,
  • Sahil S. Sethi,
  • Irina L. Grigorova

DOI
https://doi.org/10.3389/fimmu.2018.02044
Journal volume & issue
Vol. 9

Abstract

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Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.

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