Marker-assisted selection strategies for developing resistant soybean  plants to cyst nematode

Fernanda Abreu Santana; Martha Freire da Silva; Julierme Kellen Freitas Guimarães; Marcia Flores da Silva Ferreira; Waldir Dias Pereira; Newton Deniz Piovesan; Everaldo Gonçalves de Barros

Crop Breeding and Applied Biotechnology (Oct 2014)

Marker-assisted selection strategies for developing resistant soybean plants to cyst nematode

Fernanda Abreu Santana,
Martha Freire da Silva,
Julierme Kellen Freitas Guimarães,
Marcia Flores da Silva Ferreira,
Waldir Dias Pereira,
Newton Deniz Piovesan,
Everaldo Gonçalves de Barros

Affiliations

Fernanda Abreu Santana: Universidade Estadual do Norte Fluminense Darcy Ribeiro, Laboratório de Melhoramento Genético Vegetal, 28.013-602, Campos dos Goytacazes, RJ, Brazil.
Martha Freire da Silva: Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuária, 36.570-900, Viçosa, MG, Brazil
Julierme Kellen Freitas Guimarães: Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuária, 36.570-900, Viçosa, MG, Brazil
Marcia Flores da Silva Ferreira: Universidade Federal do Espírito Santo, Alto Universitário, S/N, 29.500-000, Alegre, ES, Brazil
Waldir Dias Pereira: Embrapa Soja, C. P. 231, 86.001-970, Londrina, PR, Brazil
Newton Deniz Piovesan: Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuária, 36.570-900, Viçosa, MG, Brazil
Everaldo Gonçalves de Barros: Universidade Católica de Brasília, 70.790-160, Brasília, DF, Brazil

Journal volume & issue: Vol. 14, no. 3
pp. 180 – 186

Abstract

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Resistant lines can be identified by marker-assisted selection (MAS), based on alleles of genetic markers linked to the resistance trait. This reduces the number of phenotypically evaluated lines, one of the limitations in the development of cultivars with resistance to soybean cyst nematode (SCN). This study evaluated the efficiency of microsatellites near quantitative trait loci (QTL) for SCN resistance, in the linkage groups (LG) G and A2 of soybean, for the selection of resistant genotypes in populations originated from crosses between the cultivars Vmax and CD201. The QTL of LG A2 was not detected in ‘Vmax’ (derived from PI 88788). In MAS, the microsatellites of LG G were efficient in selecting F6:7 families with resistance and moderate resistance to SCN race 3. The selection efficiency of the microsatellites Sat_168, Satt309 and Sat_141 was greater than 93%.

Published in Crop Breeding and Applied Biotechnology

ISSN: 1518-7853 (Print); 1984-7033 (Online)
Publisher: Brazilian Society of Plant Breeding
Country of publisher: Brazil
LCC subjects: Agriculture: Plant culture; Technology: Chemical technology: Biotechnology
Website: http://cbab.sbmp.org.br/

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