Association of TNF-α-308G/A gene polymorphism with coronavirus disease-19 severity

Abstract

Background: From the time when the first outbreak of coronavirus disease (COVID-19), only a small proportion of infected people developed a severe infection, which is usually a sequel of cytokine overproduction. Genetic variations in the genes of some cytokines can influence the transcription rate of these cytokines. Objective: The going research article tried to evaluate the link between tumor necrosis factor (TNF)-α-308 gene polymorphism and COVID-19 severity. Materials and Methods: Blood samples were obtained from 60 patients with COVID-19 and verified by reverse transcriptase polymerase chain reaction (PCR) in nasopharyngeal swabs. Patients were categorized into two categories based on the severity of the disease: severe COVID-19 included 30 patients and mild/moderate COVID-19 with 30 patients. The nucleic DNA was obtained from the whole blood, and TNF-α-308G>A polymorphism was genotyped utilizing PCR-restriction fragment length polymorphism. Results: Homozygous (GG) and heterozygous (GA) genotypes were more frequent among severe than among mild cases, although the differences were not significant. At the allelic level, the frequency of a mutant allele (A) was higher in severe than in mild cases with a noticeable distinction (odds ratio = 2.49, 95% confidence interval = 1.1–5.64, P = 0.029). Conclusion: Allele A of TNF-α-308G>A may be deemed a threat for the severity of COVID-19.

Keywords

• coronavirus disease
• restriction fragment length polymorphism
• tumor necrosis factor-alpha gene polymorphism