High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats

Dejana Rakic; Jovana Joksimovic Jovic; Vladimir Jakovljevic; Vladimir Zivkovic; Maja Nikolic; Jasmina Sretenovic; Marina Nikolic; Nikola Jovic; Marija Bicanin Ilic; Petar Arsenijevic; Aleksandra Dimitrijevic; Tatjana Vulovic; Natasa Ristic; Kristina Bulatovic; Sergej Bolevich; Lazar Stijak; Suzana Pantovic

doi:10.3390/medicina59061104

Medicina (Jun 2023)

High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats

Dejana Rakic,
Jovana Joksimovic Jovic,
Vladimir Jakovljevic,
Vladimir Zivkovic,
Maja Nikolic,
Jasmina Sretenovic,
Marina Nikolic,
Nikola Jovic,
Marija Bicanin Ilic,
Petar Arsenijevic,
Aleksandra Dimitrijevic,
Tatjana Vulovic,
Natasa Ristic,
Kristina Bulatovic,
Sergej Bolevich,
Lazar Stijak,
Suzana Pantovic

Affiliations

Dejana Rakic: Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Jovana Joksimovic Jovic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Vladimir Jakovljevic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Vladimir Zivkovic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Maja Nikolic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Jasmina Sretenovic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Marina Nikolic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Nikola Jovic: Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Marija Bicanin Ilic: Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Petar Arsenijevic: Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Aleksandra Dimitrijevic: Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
Tatjana Vulovic: University Clinical Center Kragujevac, Zmaj Jovina 30, 34000 Kragujevac, Serbia
Natasa Ristic: Department of Cytology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, 11060 Belgrade, Serbia
Kristina Bulatovic: Faculty of Medicine, University of Pristina in Kosovska Mitrovica, 38220 Kosovska Mitrovica, Serbia
Sergej Bolevich: Department of Human Pathology, I.M. Sechenov First Moscow State Medical University, 119146 Moscow, Russia
Lazar Stijak: Institute of Anatomy, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia
Suzana Pantovic: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia

DOI: https://doi.org/10.3390/medicina59061104
Journal volume & issue: Vol. 59, no. 6
p. 1104

Abstract

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Background and Objectives: Polycystic ovary syndrome (PCOS) is a frequent multifactorial endocrinopathy affecting women in the reproductive period, often associated with infertility and metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling the examination of the effects of certain drugs in order to discover the best possible therapeutic approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet (HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress. Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiol-valerate group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and EV group had a regular diet, while the EV + HFD group had HFD during the induction period of 60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances, along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone. Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions: The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.

Published in Medicina

ISSN: 1010-660X (Print); 1648-9144 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: https://www.mdpi.com/journal/medicina

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