Frontiers in Aging Neuroscience (Jul 2021)

RNF213 p.R4810K (c.14429G > A) Variant Determines Anatomical Variations of the Circle of Willis in Cerebrovascular Disease

  • Futoshi Eto,
  • Futoshi Eto,
  • Takeshi Yoshimoto,
  • Shuhei Okazaki,
  • Shuhei Okazaki,
  • Kunihiro Nishimura,
  • Shiori Ogura,
  • Shiori Ogura,
  • Eriko Yamaguchi,
  • Eriko Yamaguchi,
  • Kazuki Fukuma,
  • Satoshi Saito,
  • Kazuo Washida,
  • Masatoshi Koga,
  • Kazunori Toyoda,
  • Takaaki Morimoto,
  • Takaaki Morimoto,
  • Hirofumi Maruyama,
  • Akio Koizumi,
  • Akio Koizumi,
  • Masafumi Ihara

DOI
https://doi.org/10.3389/fnagi.2021.681743
Journal volume & issue
Vol. 13

Abstract

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IntroductionDysregulation of the RING finger protein 213 (RNF213) gene impairs vascular formation in experimental animal models. In addition, vascular abnormalities in the circle of Willis are associated with cerebrovascular disease. Here, we evaluated the relationship between the East Asian founder variant RNF213 p.R4810K and consequent anatomical variations in the circle of Willis in cerebrovascular disease.Patients and MethodsThe present study is an observational cross-sectional study. It included patients with acute anterior circulation non-cardioembolic stroke admitted to our institution within 7 days of symptom onset or last-known-well from 2011 to 2019, and those who participated in the National Cerebral and Cardiovascular Center Biobank. We compared anatomical variations of the vessels constituting the circle of Willis between RNF213 p.R4810K (c.14429G > A) variant carriers and non-carriers using magnetic resonance angiography and assessed the association between the variants and the presence of the vessels constituting the circle of Willis. Patients with moyamoya disease were excluded.ResultsFour hundred eighty-one patients [146 women (30%); median age 70 years; median baseline National Institutes of Health Stroke Scale score 5] were analyzed. The RNF213 p.R4810K variant carriers (n = 25) were more likely to have both posterior communicating arteries (PComAs) than the variant non-carriers (n = 456) (56% vs. 13%, P < 0.01). Furthermore, variant carriers were less likely to have an anterior communicating artery (AComA) than non-carriers (68% vs. 84%, P = 0.04). In a multivariate logistic regression analysis, the association of RNF213 p.R4810K variant carriers with the presence of both PComAs and the absence of AComA remained significant.ConclusionOur findings suggest that the RNF213 p.R4810K variant is an important factor in determining anatomical variations in the circle of Willis.

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