Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Jun 2000)

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals

  • R. Carvalho,
  • L.M.F. Passos,
  • A.M.G. Gouvea,
  • M. Resende,
  • A.S. Martins,
  • G.C. Franco

DOI
https://doi.org/10.1590/S0102-09352000000300003
Journal volume & issue
Vol. 52, no. 3
pp. 200 – 207

Abstract

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An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.

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