Frontiers in Cell and Developmental Biology (Mar 2024)
Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
- Peipei Liu,
- Peipei Liu,
- Peipei Liu,
- Peipei Liu,
- Peipei Liu,
- Ziniu Li,
- Ziniu Li,
- Ziniu Li,
- Ziniu Li,
- Ziniu Li,
- Qiuyuan Zhang,
- Qiuyuan Zhang,
- Qiuyuan Zhang,
- Qiuyuan Zhang,
- Qiuyuan Zhang,
- Jiao Qiao,
- Jiao Qiao,
- Jiao Qiao,
- Jiao Qiao,
- Jiao Qiao,
- Chenjun Zheng,
- Chenjun Zheng,
- Chenjun Zheng,
- Chenjun Zheng,
- Chenjun Zheng,
- Wenping Zheng,
- Wenping Zheng,
- Wenping Zheng,
- Wenping Zheng,
- Wenping Zheng,
- Hongyu Zhang,
- Hongyu Zhang,
- Hongyu Zhang,
- Hongyu Zhang,
- Hongyu Zhang
Affiliations
- Peipei Liu
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Peipei Liu
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Peipei Liu
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Peipei Liu
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Peipei Liu
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Ziniu Li
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Ziniu Li
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Ziniu Li
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Ziniu Li
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Ziniu Li
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Qiuyuan Zhang
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Qiuyuan Zhang
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Qiuyuan Zhang
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Qiuyuan Zhang
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Qiuyuan Zhang
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Jiao Qiao
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Jiao Qiao
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Jiao Qiao
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Jiao Qiao
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Jiao Qiao
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Chenjun Zheng
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Chenjun Zheng
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Chenjun Zheng
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Chenjun Zheng
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Chenjun Zheng
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Wenping Zheng
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Wenping Zheng
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Wenping Zheng
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Wenping Zheng
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Wenping Zheng
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- Hongyu Zhang
- National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei, China
- Hongyu Zhang
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China
- Hongyu Zhang
- China-Australia Joint Research Centre for Horticultural and Urban Pests, Huazhong Agricultural University, Wuhan, Hubei, China
- Hongyu Zhang
- Institute of Urban and Horticultural Entomology, Huazhong Agricultural University, Wuhan, Hubei, China
- Hongyu Zhang
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China
- DOI
- https://doi.org/10.3389/fcell.2024.1356151
- Journal volume & issue
-
Vol. 12
Abstract
Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species.
Keywords
