Acetylated Proteomics of UV-B Stress-Responsive in Photosystem II of <i>Rhododendron chrysanthum</i>

Abstract

Rhododendron chrysanthum (Rhododendron chrysanthum Pall.), an alpine plant, has developed UV-B resistance mechanisms and has grown to be an important plant resource with the responsive capacity of UV-B stress. Our study uses acetylated proteomics and proteome analysis, together with physiological measurement, to show the Rhododendron chrysanthum seedling’s reaction to UV-B stress. Following a 2-day, 8-h radiation therapy, 807 significantly altered proteins and 685 significantly altered acetylated proteins were discovered. Significantly altered proteins and acetylated proteins, according to COG analysis, were mostly engaged in post-translational modification, protein turnover, and chaperone under UV-B stress. It indicates that protein acetylation modification plays an important role in plant resistance to UV-B. The experimental results show that photosynthesis was inhibited under UV-B stress, but some photosynthetic proteins will undergo acetylation modification, which can alleviate the UV-B damage of plants to a certain extent. These results will serve as the basis for more research into the intricate molecular mechanisms underlying plant UV-B adaptation.

Keywords

• <i>Rhododendron chrysanthum</i> Pall.
• acetylated proteomics
• UV-B stress
• photosystem II