Field Evaluation of Recombinant Antigen ELISA in Detecting Zoonotic Schistosome Infection Among Water Buffaloes in Endemic Municipalities in the Philippines

Jose Ma. M. Angeles; Jose Ma. M. Angeles; Yasuyuki Goto; Masashi Kirinoki; Elena A. Villacorte; Kharleezelle J. Moendeg; Kharleezelle J. Moendeg; Pilarita T. Rivera; Yuichi Chigusa; Shin-ichiro Kawazu

doi:10.3389/fvets.2020.592783

Frontiers in Veterinary Science (Oct 2020)

Field Evaluation of Recombinant Antigen ELISA in Detecting Zoonotic Schistosome Infection Among Water Buffaloes in Endemic Municipalities in the Philippines

Jose Ma. M. Angeles,
Jose Ma. M. Angeles,
Yasuyuki Goto,
Masashi Kirinoki,
Elena A. Villacorte,
Kharleezelle J. Moendeg,
Kharleezelle J. Moendeg,
Pilarita T. Rivera,
Yuichi Chigusa,
Shin-ichiro Kawazu

Affiliations

Jose Ma. M. Angeles: Department of Parasitology, College of Public Health, University of the Philippines, Manila, Philippines
Jose Ma. M. Angeles: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
Yasuyuki Goto: Laboratory of Molecular Immunology, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Masashi Kirinoki: Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan
Elena A. Villacorte: Department of Parasitology, College of Public Health, University of the Philippines, Manila, Philippines
Kharleezelle J. Moendeg: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
Kharleezelle J. Moendeg: Department of Biology, School of Science and Engineering, Ateneo de Manila University, Quezon City, Philippines
Pilarita T. Rivera: Department of Parasitology, College of Public Health, University of the Philippines, Manila, Philippines
Yuichi Chigusa: Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan
Shin-ichiro Kawazu: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

DOI: https://doi.org/10.3389/fvets.2020.592783
Journal volume & issue: Vol. 7

Abstract

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In this study, we investigated the use of recombinant antigens thioredoxin peroxidase-1 (rSjTPx-1) and tandem repeat rSj1TR in evaluating the antibody positivity rates of Schistosoma japonicum infection among water buffaloes from four endemic areas in the Philippines, two municipalities with high endemicity (Calatrava, Negros Occidental and Catarman, Northern Samar) and two municipalities nearing elimination with no cases of human schistosomiasis (Talibon and Trinidad, Bohol). These recombinant antigen ELISA assays were compared with other diagnostic tests including SEA-ELISA, FECT, and fecal-based PCR. Results showed that rSj1TR-ELISA has the highest agreement with PCR in all study areas. Furthermore, significant positivity rates among water buffaloes were seen in Talibon and Trinidad, indicating that water buffaloes are maintaining the schistosome parasites in transmission areas even in the absence of human infection. Hence, serological assay using a more sensitive and specific rSj1TR-ELISA can be used for animal surveillance to prevent emergence and re-emergence of human schistosomiasis.

Published in Frontiers in Veterinary Science

ISSN: 2297-1769 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://www.frontiersin.org/journals/veterinary-science

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