Identification of Differentially Expressed lncRNAs in Response to Blue Light and Expression Pattern Analysis of Populus tomentosa Hybrid Poplar 741

Hongyan Li; Yiwen Zhang; Jinping Lan; Shijie Wang; Hongyu Cai; Xin Meng; Yachao Ren; Minsheng Yang

doi:10.3390/plants12173157

Plants (Sep 2023)

Identification of Differentially Expressed lncRNAs in Response to Blue Light and Expression Pattern Analysis of Populus tomentosa Hybrid Poplar 741

Hongyan Li,
Yiwen Zhang,
Jinping Lan,
Shijie Wang,
Hongyu Cai,
Xin Meng,
Yachao Ren,
Minsheng Yang

Affiliations

Hongyan Li: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Yiwen Zhang: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Jinping Lan: Life Science Research Center, Hebei North University, Zhangjiakou 075000, China
Shijie Wang: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Hongyu Cai: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Xin Meng: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Yachao Ren: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China
Minsheng Yang: Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, China

DOI: https://doi.org/10.3390/plants12173157
Journal volume & issue: Vol. 12, no. 17
p. 3157

Abstract

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Poplar is an important shelterbelt, timber stand, and city tree species that has been the focus of forestry research. The regulatory role of the long non-coding RNA molecule (lncRNA; length > 200 nt) has been a research hotspot in plants. In this study, seedlings of 741 poplar were irradiated with LED blue and white light, and the Illumina HiSeq 2000 sequencing platform was used to identify lncRNAs. |logFC| > 1 and p MSTRG.20413.1-ptc-miR396e-5p-GRF9, were verified using quantitative real-time polymerase chain reaction analysis, and the expression patterns were analyzed. The analysis showed that the ptc-miR396e-5p expression was downregulated, while lncRNA MSTRG.20413.1 and GRF9 expression was upregulated, after blue light exposure. These results indicate that lncRNAs interact with miRNAs to regulate gene expression and affect plant growth and development.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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