Inherently confinable split-drive systems in Drosophila

Gerard Terradas; Anna B. Buchman; Jared B. Bennett; Isaiah Shriner; John M. Marshall; Omar S. Akbari; Ethan Bier

doi:10.1038/s41467-021-21771-7

Nature Communications (Mar 2021)

Inherently confinable split-drive systems in Drosophila

Gerard Terradas,
Anna B. Buchman,
Jared B. Bennett,
Isaiah Shriner,
John M. Marshall,
Omar S. Akbari,
Ethan Bier

Affiliations

Gerard Terradas: Section of Cell and Developmental Biology, University of California, San Diego
Anna B. Buchman: Section of Cell and Developmental Biology, University of California, San Diego
Jared B. Bennett: Biophysics Graduate Group, Division of Biological Sciences, College of Letters and Science, University of California
Isaiah Shriner: Section of Cell and Developmental Biology, University of California, San Diego
John M. Marshall: Divisions of Epidemiology and Biostatistics, School of Public Health, University of California
Omar S. Akbari: Section of Cell and Developmental Biology, University of California, San Diego
Ethan Bier: Section of Cell and Developmental Biology, University of California, San Diego

DOI: https://doi.org/10.1038/s41467-021-21771-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

NHEJ alleles and Cas9 remnants after a gene drive introduction are scientific and public concerns. Here, the authors use split drives with recoded rescue elements to target essential genes and minimize the appearance of NHEJ alleles while also leaving no trace of Cas9.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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