Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme

Abstract

Objective To investigate the expression and clinical significance of ZMYND11 in glioblastoma multiforme(GBM). Methods The tumor samples(tumor group) and non-neoplasm brain tissue(control group) were collected intraoperatively from 20 cases of GBM and 20 patients with severe brain injury in the Second Hospital of Hebei Medical University. The difference of ZMYND11 expression between two groups was determined using qRT-PCR and Western blot. U87 cells with upregulated ZMYND11 expression was constructed using transfection of ZMYND11-overexpression lentivirus. CCK, Transwell assay and flow cytometry were performed to identify the role of ZMYND11 in the proliferation, invasion and apoptosis of U87 cells. U87 cells with ZMYND11 overexpression were inoculated into nude mice for in vivo experiment. Results The expression levels of ZMYND11 mRNA and protein were lower in GBM tissues than those in non-neoplasm brain tissues(P < 0.001). In vitro, ZMYND11 inhibited effectively the proliferation and invasion, as well as promoted the apoptosis of U87 cells. In vivo, ZMYND11 suppressed remarkably the growth of graft tumor. Conclusion ZMYND11 inhibits the development and progression of GBM.

Keywords

• glioblastoma multiforme
• cell proliferation
• cell apoptosis
• nude mouse transplantation tumor