An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR

Zhaoqi Kang; Manman Zhang; Kaiyu Gao; Wen Zhang; Wensi Meng; Yidong Liu; Dan Xiao; Shiting Guo; Cuiqing Ma; Chao Gao; Ping Xu

doi:10.1038/s41467-021-23723-7

Nature Communications (Jun 2021)

An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR

Zhaoqi Kang,
Manman Zhang,
Kaiyu Gao,
Wen Zhang,
Wensi Meng,
Yidong Liu,
Dan Xiao,
Shiting Guo,
Cuiqing Ma,
Chao Gao,
Ping Xu

Affiliations

Zhaoqi Kang: State Key Laboratory of Microbial Technology, Shandong University
Manman Zhang: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Department of Radiobiology, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College
Kaiyu Gao: State Key Laboratory of Microbial Technology, Shandong University
Wen Zhang: Center for Gene and Immunotherapy, The Second Hospital of Shandong University
Wensi Meng: State Key Laboratory of Microbial Technology, Shandong University
Yidong Liu: State Key Laboratory of Microbial Technology, Shandong University
Dan Xiao: State Key Laboratory of Microbial Technology, Shandong University
Shiting Guo: State Key Laboratory of Microbial Technology, Shandong University
Cuiqing Ma: State Key Laboratory of Microbial Technology, Shandong University
Chao Gao: State Key Laboratory of Microbial Technology, Shandong University
Ping Xu: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

DOI: https://doi.org/10.1038/s41467-021-23723-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

Read online

L-2-hydroxyglutarate (L-2-HG) is an important metabolite but its regulation is poorly understood. Here the authors report an L-2-HG FRET biosensor based on the allosteric transcription factor, LhgR, to monitor L-2-HG in cells and biological samples.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal