CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing

Xueni Li; Shiheng Liu; Jiansen Jiang; Lingdi Zhang; Sara Espinosa; Ryan C. Hill; Kirk C. Hansen; Z. Hong Zhou; Rui Zhao

doi:10.1038/s41467-017-01241-9

Nature Communications (Oct 2017)

CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing

Xueni Li,
Shiheng Liu,
Jiansen Jiang,
Lingdi Zhang,
Sara Espinosa,
Ryan C. Hill,
Kirk C. Hansen,
Z. Hong Zhou,
Rui Zhao

Affiliations

Xueni Li: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus
Shiheng Liu: Electron Imaging Center for Nanomachines University of California, Los Angeles (UCLA)
Jiansen Jiang: Electron Imaging Center for Nanomachines University of California, Los Angeles (UCLA)
Lingdi Zhang: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus
Sara Espinosa: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus
Ryan C. Hill: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus
Kirk C. Hansen: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus
Z. Hong Zhou: Electron Imaging Center for Nanomachines University of California, Los Angeles (UCLA)
Rui Zhao: Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus

DOI: https://doi.org/10.1038/s41467-017-01241-9
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

Read online

U1 snRNP is critical for 5′ splicing site recognition in pre-mRNA splicing. Here the authors describe the cryo-EM structure of the yeast U1 snRNP and suggest that PrpF39 is an alternative splicing factor essential for the successful recruitment of U1 snRNP by other alternative splicing factors.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal