Cancer Management and Research (Oct 2020)
LncRNA UCA1 Affects the Cell Proliferation, Migration, Invasion and Apoptosis of Hepatic Carcinoma Cells by Targeting MicroRNA-193a-3p
Abstract
Hong-Zhen Wang,1 Li Liu,2 Yan Xu,1 Guang-Ye Zhang,3 Yan-Yan Wang2 1Department of Oncology, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People’s Republic of China; 2Department of ENT, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People’s Republic of China; 3Department of Hepatology, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People’s Republic of ChinaCorrespondence: Yan-Yan WangDepartment of ENT, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People’s Republic of ChinaTel +86-13906331429Email [email protected]/Background: Hepatic carcinoma (HCC) is the fourth lethal cancer in the world, but its relationship with lncRNA urothelial cancer-associated 1 (UCA1)/microRNA-193a-3p axis remains unclear, so this study would explore the relationship.Methods: A real-time polymerase chain reaction (RT-PCR) assay was carried out to quantify lncRNA UCA1 and microRNA-193a-3p in HCC tissues and cells, and relevant overexpression or inhibition vectors were constructed to analyze the influences of lncRNA UCA1 and microRNA-193a-3p on HCC cells. A Transwell assay was used to measure invasion and migration of HCC cells, and a Western blot assay to quantify protein biomarkers of apoptosis, invasion, and migration, a MTT assay to determine cell viability, a flow cytometry to detect cell cycle, and a dual-luciferase reporter gene assay to analyze the correlation between lncRNA UCA1 and microRNA-193a-3p.Results: LncRNA UCA1 was increased in HCC, while microRNA-193a-3p was decreased. Down-regulated lncRNA UCA1 could up-regulate microRNA-193a-3p, and down-regulated lncRNA UCA1 or up-regulated microRNA-193a-3p would strengthen cell apoptosis and weaken cell migration, invasion, and proliferation. Furthermore, lncRNA UCA1 could negatively regulate microRNA-193a-3p by binding to it.Conclusion: LncRNA UCA1 promotes malignant hyperproliferation of HCC cells by repressing microRNA-193a-3p.Keywords: hepatic carcinoma, lncRNA UCA1/microRNA-193a-3p, cell carcinogenesis
