Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Huyen Thi Lam Nguyen; Alice Soragni

STAR Protocols (Sep 2020)

Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Huyen Thi Lam Nguyen,
Alice Soragni

Affiliations

Huyen Thi Lam Nguyen: Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA
Alice Soragni: Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute (MBI), University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center (JCCC), University of California, Los Angeles, Los Angeles, CA, USA; California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 2
p. 100056

Abstract

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Summary: Tumor organoids are promising tools for cancer biology investigations and pre-clinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings.For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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